Links to Regulations and Guidance US Department Of Transportation (DOT) Overview of substance abuse testing for commercial drivers and other regulated safety-sensitive employees Federal Motor Carrier Safety Administration (FMCSA) FMCSA drug testing regulations 49 CFR Part 382Federal Motor Carrier Safety Administration (FMCSA) Employer ResourcesMotor Carrier Clearinghouse Federal Transit Administration (FTA) FTA drug testing regulations 49 CFR Part 655